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DNA Fragment Separation By Gel Electrophoresis

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Several DNA samples were digested with various restriction enzymes (endonucleases) and separated via gel electrophoresis, as shown in the following diagram.

Diagram of a vertical gel electrophoresis result with three labeled lanes at the top: EcoRI, HaeIII, and HindIII. Each lane contains horizontal black bands representing DNA fragments. The EcoRI lane h

Which of the following accounts for the distinct banding patterns observed in the different lanes?

A

Differences in the chemical buffers applied to the individual lanes

B

Differences in the terminal configurations of the fragments, such as blunt versus sticky ends

C

Differences in the electrical voltage applied to the individual lanes

D

Differences in the sizes of the DNA fragments among the samples

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